Hyaluronic Acid Derived from Other Streptococci Supports Streptococcus pneumoniae In Vitro Biofilm Formation

Table 1

Oligonucleotides designed from Streptococcus pneumoniae strain R6 genome and used in this study.

Gene	Locus	Primer sequences	Amplicon size in base pair
hysA	spr0286	TGTCGTTCGAACAGTCAGGG	120 bp
		TTGTTGATGGCACGAGGTGT
ugl	spr0292	ATCACCATGATCTCGGCTTC	97 bp
		GCAGCTTTCAAGGTTGCTTC
PTS-EIIA	spr0291	TCTCGGGTTTGAACTTAGCCA	96 bp
		GGGCCGCCACTACTGATTTA
nanA	spr1536	CCAATGCTTCAAATGGTCAG	120 bp
		TATAGAATGCTGGGGCCTTG
nanB	spr1531	ATTGGCTCACTCCACGTTTT	94 bp
		ATGCACGTTATGGTGGGACT
bgaA	spr0565	CAAGCCAGCCGTGAACGCTATAAGG	128 bp
		GAGTGGGCAGTCAGGGTGAATTTCC
strH	spr0057	GGTTTTTCCGTTGGCAGTAA	121 bp
		GCTCAAACGCATCGTAGACA
lytA	spr1754	CGTCCCAGGCACCATTATCA	95 bp
		CTGGCGGAAAGACCCAGAAT
comA	spr0043	GAGACGCGAGCCATTAAGG	156 bp [20]
		GGGATCTGGATCGGCAATATGA
luxS	spr0308	TATGTTCGCTTGATTGGG	105 bp
		GCCGGCAGTAGGGATAGAGT
gyrB	spr1099	CAGATCAAGAAATCAAACTCCAA	171 bp [20]
		CAGCATCATCTACAGAAACTC