Isolation of Small SSEA-4-Positive Putative Stem Cells from the Ovarian Surface Epithelium of Adult Human Ovaries by Two Different Methods

<table>SSEA-4, OCT4A, and SOX-2-positivity of small, round cells isolated by MACS or FACS from the populations of cells retrieved by the ovarian cortex biopsy (ovarian surface epithelium) brushings, as revealed by immunocytochemistry. For SSEA-4, (a1) Merged; (a2) SSEA-4 (green) staining; (a3) DAPI (blue) staining of the cell nuclei. For OCT4A, (b1) Merged; (b2) OCT4A (green) staining; (b3) DAPI (blue) staining of the cell nuclei. For SOX-2, (c1, d1) Merged; (c2, d2) SOX-2 (red) staining; (c3, d3) DAPI (blue) staining of the cell nuclei (fluorescence microscope). <i>Red Bar</i>: 10 <i>μ</i>m.</table>

BioMed Research International

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Figure 6

Figure 6: Isolation of Small SSEA-4-Positive Putative Stem Cells from the Ovarian Surface Epithelium of Adult Human Ovaries by Two Different Methods 