Research Article
Isolation of Small SSEA-4-Positive Putative Stem Cells from the Ovarian Surface Epithelium of Adult Human Ovaries by Two Different Methods
Table 1
The expression of genes related to pluripotency (OCT4A) and oocytes (c-KIT, DMC1, FIGLA, SCP3, VASA, ZP1, ZP2, and ZP3) in twelve oocyte-like cells (OLC1–12) developed in vitro and analyzed by single-cell RT-PCR. The OLCs were compared to three nonfertilized mature (MII) oocytes (OOCYTE1–3) from the in vitro fertilization programme (positive control) and to 5 samples of human chondrocytes (H1–5; negative control). Eleven OLCs expressed the gene OCT4A, two OLCs the gene ZP3, one OLC the gene SCP3, and one OLC the gene c-KIT. The oocytes expressed all analyzed genes, while chondrocytes did not express any of them.
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