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BioMed Research International
Volume 2013 (2013), Article ID 690491, 12 pages
http://dx.doi.org/10.1155/2013/690491
Research Article

The Therapeutic Potential of Umbilical Cord Mesenchymal Stem Cells in Mice Premature Ovarian Failure

1Blood Transfusion Department, the General Hospital of the People’s Liberation Army, Beijing 100853, China
2Department of Obstetrics and Gynecology, First Affiliated Hospital of the General Hospital of the People’s Liberation Army, Beijing 100048, China
3Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China
4Department of Obstetrics and Gynecology, the General Hospital of the People’s Liberation Army, Beijing 100853, China
5Tissue Engineering Research Center of Academy of Military Medical Sciences, Beijing 100850, China

Received 26 April 2013; Revised 25 June 2013; Accepted 11 July 2013

Academic Editor: Elena Jones

Copyright © 2013 Shufang Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mesenchymal stem cells, which are poorly immunogenic and have potent immunosuppressive activities, have emerged as promising cellular therapeutics for the treatment of several diseases. Mesenchymal-like cells derived from Wharton’s Jelly, called umbilical cord matrix stem cells (UCMSCs), reportedly secrete a variety of cytokines and growth factors, acting as trophic suppliers. Here, we used UCMSCs to treat premature ovarian failure (POF). Ovarian function was evaluated by ovulation and the number of follicles. Apoptosis of the granulosa cells (GC) was analyzed by TUNEL staining. We found that after transplantation of the UCMSCs, apoptosis of cumulus cells in the ovarian damage model was reduced and the function of the ovary had been recovered. The sex hormone level was significantly elevated in mice treated with UCMSCs. The number of follicles in the treated group was higher than in the control group. Our results demonstrate that UCMSCs can effectively restore ovary functionality and reduce apoptosis of granulosa cells. We compared the RNA expression of the UCMSCs treated group with the POF model and wild-type control group and found that the UCMSC group is most similar to the wild-type group. Our experiments provide new information regarding the treatment of ovarian function failure.