Figure 6: Lung macrophages from STAT6−/− and STAT6+/+T. canis-infected mice display different phenotypes. Macrophages were obtained at different time points after infection and analyzed by flow cytometry for the detection of different cell markers associated with alternative activation of macrophages. (a) F4/80+MR+ lung cells, (b) F4/80+Gr1+ lung cells, and (c) F4/80+PD-L1+ lung cells, as described in Section 2. Data are expressed as the mean SE and are representative of two independent experiments, . comparing STAT6−/− versus STAT6+/+ at the same time point. ND: not determined.