Gene Therapy of Multiple Sclerosis Using Interferon β-Secreting Human Bone Marrow Mesenchymal Stem Cells
Figure 6
The effect of MSCs-IFNβ on migration and BBB permeability in EAE mice. (a) NIR-labeled MSCs-IFNβ detected in the brain and spinal cord of EAE or normal mice at day 7 after treatment (/each group). Mouse images show the biofluorescence signal from the brain and spinal cord detected in one representative animal. The biofluorescence signal is given as photons per second. For the detection of the NIR-positive cells (red) in the brain (bottom-left panel) and spinal cord (bottom-right panel) tissues, cryosections from EAE mouse were stained by human nuclear antigen (hNA). hNA-positive nuclei (green) were stained, and counterstaining was conducted with DAPI (blue). Magnification: ×200. (b) The integrity of BBB of different treated animals was detected by quantitative measurement for EB content at day 7 after treatment (/each group). The intensity of EB in the brain and spinal cord tissue was measured at 620 nm using a spectrofluorometer. Columns: mean; bars: SE. *; **; Student’s t-test. The results are representative of three independent experiments.
