High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis
Figure 3
Extracellular matrix production. Histology of the HDS stained with Picro Sirius Red (red) in row 1 detecting collagen and AbvG (blue) in row 2 detecting proteoglycans. (a) HDS in Defined medium at 1 week. (b) HDS in Defined medium at 6 weeks. (c) HDS in Cardiac medium at 9 weeks (Defined medium for 6 weeks followed by Cardiac medium for 3 weeks) and (d) in Endothelial medium at 4 weeks (Defined medium for 1 week followed by Endothelial medium for 3 weeks). The multilayer area and the buds are marked by arrows.
