High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis
Figure 7
Activities within the progenitor cell regulating Notch and Wnt pathways. (a) Relative mRNA expression of the receptor NOTCH1, its ligand JAG1, and downstream gene HEY2. Early and late time points of HDS cultured in Defined media were compared either to HDS cultured in Cardiac () or Endothelial () media. (b) Staining with antibody against NOTCH1 (red) at 6 weeks in HDS cultured in Defined medium. (c) When cultured in Cardiac medium, NOTCH1 staining was mostly located to the additional multilayer area (arrow) at 9 weeks. (d) Active form of -catenin (red) was detected in many cells in the HDS, illustrated by the nuclear position of the staining (arrows). DAPI (blue) was used to counterstain nuclei. (e) Relative mRNA expression of ISL1 and DKK1 after one week of stimulation with GSK inhibitor SB216763, DMSO as negative control ().
