Characterization of C-S Lyase from C. diphtheriae: A Possible Target for New Antimicrobial Drugs
Figure 2
CD and fluorescence emission spectra of C-S lyase in the presence of substrates and substrate analogues. (a) CD spectra of enzyme (solid line) in the presence of 450 mM L-homoserine (dotted line) and 150 mM L-methionine (dashed line) in 50 mM Tris-HCl pH 8.5. (b) Fluorescence emission spectra of 1 μM enzyme (solid line) in the presence of 150 mM L-methionine (dotted line), 100 mM L-serine (dashed line), and 100 mM O-acetyl-L-serine (dash dotted line) upon excitation at 295 nm. Inset: emission spectra of 10 μM enzyme (solid line) in the presence of 100 mM O-acetyl-L-serine (dash dotted line), 100 mM L-serine (dashed line), and 150 mM L-methionine (dotted line) upon excitation at 418 nm. All spectra are recorded in 20 mM Bis-Tris-propane pH 8.5.
