Characterization of C-S Lyase from C. diphtheriae: A Possible Target for New Antimicrobial Drugs

Table 2

Kinetic parameters for C-S lyase wt and mutants using L-Cth in 50 mM MOPS, and 50 mM bicine, 50 mM proline pH 9.0 at 30°C.

Protein	𝐾𝑚; (mM)	𝑘 cat; (s^−1)	Relative activity (%)
wt	1.07±0.22	92.67±4.44	100
Y114F	0.20±0.01	1.20±0.02	1.3
Y55F	0.62±0.21	0.45±0.01	0.5
R351A	n.d.*	n.d.*	—

*n.d.: not detected.