BioMed Research International

Research Article

Unraveling the Lipolytic Activity of Thermophilic Bacteria Isolated from a Volcanic Environment

Table 1

Comparison of the lipolytic activities among the 101 thermophilic bacterial isolates in Rhodamine olive oil agar (ROA) and olive oil liquid (OLM) cultures.
StrainROAOLM
24 h48 h48 h72 h
SP1−−−−
SP2−−0.0400.063
SP3−−−−
SP4−+0.0620.102
SP5−−−−
SP6++0.0580.073
SP7−−0.2500.085
SP8−−0.2500.270
SP9−−0.3070.167
SP10−−0.5000.200
SP11−−0.3580.217
SP12−−0.3500.142
SP13−−0.5830.175
SP14 − − 1.070 2.367
SP15−−0.1080.147
SP16−−0.0220.065
SP17−−−−
SP18−−0.2000.067
SP19−−0.2170.248
SP20−−0.1530.055
SP21−−0.5500.427
SP22 − − 1.618 0.450
SP23−−0.5670.192
SP24−−0.2170.133
SP25−+0.0440.092
SP26−−−−
SP27++0.1500.288
SP28−−−−
SP29 − − 0.967 0.620
SP30−+0.4221.027
SP31−−−−
SP32−+0.0110.085
SP33−−−−
SP34−−−−
SP35−−0.0950.258
SP36−−−−
SP37−−−−
SP38−−0.1780.767
SP39−−−−
SP40−−0.0630.115
SP41−−−−
SP42−−0.4500.192
SP43−−0.1200.072
SP44−−0.1200.123
SP45−−0.1530.088
SP46−−−−
SP47−−0.0380.045
SP48−−0.0430.027
SP49−−0.0330.032
SP50−−0.0420.037
SP51−−0.0580.032
SP52−−−−
SP53−−−−
SP54−−0.0370.045
SP55−−0.0350.062
SP56−−0.0520.000
SP57−−0.0380.183
SP58−−−−
SP59−−0.0000.085
SP60−−−−
SP61−−0.0400.037
SP62−−−−
SP63−−0.0380.053
SP64−−0.0570.000
SP65−−0.0450.043
SP66−−0.1150.047
SP67−−0.0530.075
SP68−−−−
SP69−−−−
SP70−−0.0430.070
SP71++0.0430.052
SP72−−0.2080.244
SP73 + ++ 1.083 0.740
SP74−−0.0880.000
SP75 + ++ 1.540 1.667
SP76 − − 1.297 1.317
SP77−−0.4830.228
SP78−−0.2200.150
SP79 − − 1.167 1.245
SP80++0.1370.110
SP81−−0.5430.293
SP82−+0.2350.250
SP83 + + 0.625 1.433
SP84−+0.1670.133
SP85−−0.0770.000
SP86−−0.0630.070
SP87−+0.0580.000
SP88−−−−
SP89−−−−
SP90−+0.5930.233
SP91−−0.5170.767
SP92++0.5170.522
SP93 ++ ++ 0.610 0.633
SP94−−0.5500.257
SP95−−1.0270.600
SP96−−0.0730.085
SP97−−−−
SP98−−−−
SP99−−−−
SP100−−0.4000.642
SP101−−0.0000.122
In ROA cultures, qualitative evaluation was performed based on fluorescence halo production (−: no halo; +: partial coverage of the plate; ++: full coverage of the plate). In OLM cultures, values represent the extracellular lipolytic activity in nkat·mL−1 (average of triplicate flasks: max. SD ± 9.4%). Bold/italicized lines represent those strains selected for further study.