Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of <i>Actinoplanes utahensis</i> ZJB-08196

<table>Time courses of acarbose accumulation, maltose and glucose consumption, cell growth, and impurity C formation during fed-batch cultivation of <i>A. utahensis </i>ZJB-08196 with the addition of validamine at a final concentration of 20 mg/L in fermentation medium prior to inoculation. Symbols: ■: acarbose; ▲: impurity C; •: DCW; □: maltose; ○: glucose. Fermentations were carried at 28°C and 200 rpm for 216 h. Twice feedings indicated by the arrows; feed volume 5 mL; feed composition: 14.0 g/L maltose, 6.0 g/L glucose, and 9.0 g/L soybean meal.</table>

BioMed Research International

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Figure 6

Figure 6: Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of <i>Actinoplanes utahensis</i> ZJB-08196 

Figure 6 | Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196 