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Figure 12: Images depicts the cell uptake of Nile Red loaded: (a) control, (b) C-ANC-G, (c) M-ANC-G, (d) C-HNC-G, and (e) M-HNC-G formulation treated cells taken after 2 hr (at 40x magnification). For this assay Lung Cancer H460 cells ( ) were seeded on six well plates and incubated at °C under 5% CO2 for 24 h. The cells were treated with various Nile Red loaded formulations for 1 hr. After 1 hr media was removed and the resulting cells were washed with PBS and fixed with 2% formaldehyde in the PBS at room temperature for 10 min, followed by washing with PBS twice. The samples were imaged using Axiovert 40 inverted microscope (magnification 40x) with Nile Red 554 nm excitation and 635 nm emission wavelength with mercury vapor lamp as light source for fluorescence imaging. Arrow head indicates cells with marked fluorescence.