712678.fig.002a
(a)
712678.fig.002b
(b)
712678.fig.002c
(c)
Figure 2: Optimization of (a) BSA concentration (% w/v), concentration of diluted ethanol (% v/v), and (c) volume of BSA solution to total ethanol ratio (v/v). Albumin concentration was optimized between 1% and 7% w/v (at constant volume of BSA solution to total ethanol ratio 1 : 1 v/v; concentration of diluted ethanol 100% v/v), while the effect of volume of BSA solution to total ethanol ratio on nanoparticle formulation was studied between 1 : 00 and 1 : 1.60 v/v (at albumin concentration, 1% w/w; concentration of diluted ethanol, 100% v/v). The concentration of diluted ethanol was optimized between 30% and 100% v/v keeping other variables constant (albumin concentration 1% w/v; volume of BSA solution to total ethanol ratio 1 : 1 v/v). Dotted square box represents the selection window for various levels engaged for Taguchi experiment design. Results are represented as mean SD, .