Table 2: Experimental data values for particle size and entrapment of albumin nanoparticles obtained by Taguchi orthogonal array table of L9 Design.

Albumin Concentration (% w/v)Volume of BSA solution to total ethanol ratio (v/v)Concentration diluted ethanol (% v/v)Size (nm)Entrapment efficiency (%)

31 : 0.75100
31 : 0.9070
31 : 1.0540
41 : 0.7570
41 : 0.9040
41 : 1.05100
51 : 0.7540
51 : 0.90100
51 : 1.0570

Particle size and surface charge (zeta potential) were determined in PBS at 25°C by dynamic light scattering using a NICOMP ZLS 380 analyzer (PSS-NICOMP, Santa Barbara, CA, USA) [8]. Entrapment efficiency was determined by employing Vivaspin 500 ultracentrifuge filters with molecular cut-off weight of 300 K Da (Viva Products, Inc., Littleton, MA, USA). Aqueous filtrate generated at the bottom of Vivaspin 500 ultracentrifuge tubes was subjected to UV spectrophotometric analysis to determine the concentration of GEM ( 248.6 nm; = 0.7053 × 104 L mol−1 cm−1) [13].
Results are presented as mean ± SD, .