Persistent Organic Pollutants Induced Protein Expression and Immunocrossreactivity by Stenotrophomonas maltophilia PM102: A Prospective Bioremediating Candidate
Figure 7
(a) Western blot with total antibody against intracellular proteins of PM102. First lane shows reaction against proteins from PM102 grown in 0.2% TCE + 0.1% peptone; second lane shows total antibody response against proteins extracted from PM102 isolate grown in 0.2% glucose with 0.1% peptone; third lane shows reaction against proteins from PM102 grown in 0.2% benzene and 0.1% peptone; fourth lane shows reaction of total antibody against proteins from PM102 grown with 0.2% toluene + 0.1% peptone; the last lane shows antibody response against proteins of PM102 grown in 0.2% chloroform with 0.1% peptone. (b) Western blot with antibody preadsorbed in proteins from PM102 grown in peptone. The preadsorbed antibody reacted against a single 35.14 kDa protein from PM102 grown with TCE, benzene, toluene, or chloroform. No reaction was seen against proteins from PM102 grown in glucose.
