Alantolactone Induces Apoptosis in HepG2 Cells through GSH Depletion, Inhibition of STAT3 Activation, and Mitochondrial Dysfunction

Figure 3

Flow cytometry analysis of apoptosis in HepG2 cells treated with 40 μM alantolactone for various time points. (a) Control, ((b), (c) and (d)) cells were treated with 40 μM alantolactone for 3, 6, and 12 h, respectively. (e) cells were treated with 40 μM alantolactone in the presence of 3 mM NAC for 12 h. (f) Data are expressed as mean ± SD (𝑛=3). Columns not sharing the same superscript letter differ significantly (𝑃<0.05).

(a) a = control 

(b) b = 3 h

(c) c = 6 h

(d) d = 12 h

(e) e = 12 h +NAC

(f)