Figure 4: Flow cytometry analysis of ROS generation in control and alantolactone-treated HepG2 cells. (a) Control, ((b), (c) and (d)) cells were treated with 40 μM alantolactone for 3, 6, and 12 h, respectively. After treatment, cells were incubated with DCFH-DA for 30 min at 37°C, washed with PBS, and analyzed for DCF fluorescence by flow cytometry. (e) Data are expressed as mean ± SD (). Columns not sharing the same superscript letter differ significantly ().