Figure 5: Flow cytometry analysis of MMP in control and alantolactone-treated HepG2 cells. (a) Control, (b), (c) and (d) cells were treated with 40 μM alantolactone for 3, 6, and 12 h, respectively. After treatment, cells were incubated with Rhodamine 123 for 30 min in dark, washed with PBS, and analyzed for MMP by flow cytometry. (e) Data are expressed as mean ± SD (). Columns not sharing the same superscript letter differ significantly ().