Fermentative Succinate Production: An Emerging Technology to Replace the Traditional Petrochemical Processes

Table 2

Overview of metabolic engineering strategies to improve succinate production using different microorganisms.

Strains	Genotypes	Culture strategies	Succinate production	References
Escherichia coli
SBS110MG	ΔadhE, ΔldhA, expression of Lactococcus lactis pyc	Batch	15.6 g/L, 1.3 mol/mol glucose	[32]
NZN111	ΔpflB, ΔldhA	Fed-batch	28.2 g/L, 1.13 mol/mol glucose	[33]
AFP111	ΔldhA, ΔptsG	Batch	0.88 mol/mol glucose	[34]
W3110GFA	ΔptsG, ΔpykF, ΔpykA	Batch	17.35 mM	[35]
QZ1111	ΔptsG, ΔpoxB, Δpta, ΔsdhA, ΔiclR	Batch	1.45 mmol/(g·h)	[36]
HL27659k	ΔsdhAB, ΔackA-pta, ΔpoxB, ΔiclR, ΔptsG	Fed-batch	0.91 mol/mol glucose	[37]
KJ073	ΔldhA, ΔadhE, ΔackA, ΔfocA-pflB, ΔmgsA, ΔpoxB	Batch	1.2 mol/mol glucose	[38]
JCL1208	overexpression of native ppc	Batch	10.7 g/L	[39]
xz320	ΔackA, ΔldhA, ΔadhE, ΔpflB, ΔmgsA, ΔpoxB, Δppc	Fed-batch	348 mM	[40]
LS1	ΔldhA, overexpression of native mae	Batch	2.34 g/L	[41]
NZN111	ΔpflB, ΔldhA, overexpression of native mdh	Fed-batch	15.2 g/L	[42]
NZN111	ΔpflB, ΔldhA, overexpression of native mae and fum	Batch	7 g/L	[43]
SBS990MG	ΔadhE, ΔldhA, Δpta-ackA, expression of Lactococcus lactis pyc	Batch	1.7 mol/mol glucose	[44]
NZN111	ΔpflB, ΔldhA, overexpression of native pncB	Fed-batch	18.3 g/L	[45]
K-12ppc	Δppc, expression of Actinobacillus succinogenes pck	Batch	26.4 mM	[46]
Saccharomyces cerevisiae
AH22ura3	Δsdh1, Δsdh2, Δidh1, Δidp1	Batch	3.62 g/L, 0.11 mol/mol glucose	[16]
DFRDS	ΔOSM1, ΔFRDS	Batch	130 μmol/(h·g dry cells)	[47]
8D	Δsdh, Δser3/ser33, Overexpression of native icl1	Batch	0.9 g/L	[48]
Corynebacterium glutamicum
BL-1	Δsdh, Δpta-ackA, Δpqo, Δcat, overexpression of native ace, pyc and ppc	Fed-batch	10.6 g/L, 0.45 mol/mol glucose	[17]
BOL-2	Δcat, Δpqo, Δpta-ackA, ΔldhA, overexpression of native pyc and Mycobacterium vaccae fdh	Fed-batch	1134 mM, 1.67 mol/mol glucose	[49]
Mannheimia succiniciproducens
LPK7	ΔldhA, ΔpflB, Δpta, ΔackA	Fed-batch	52.4 g/L, 1.16 mol/mol glucose	[50]