Research Article
Copper Enhanced Monooxygenase Activity and FT-IR Spectroscopic Characterisation of Biotransformation Products in Trichloroethylene Degrading Bacterium: Stenotrophomonas maltophilia PM102
Table 1
Characterisation of monooxygenase activity from Stenotrophomonas maltophilia PM102.
| Conditions for enzyme activity | Enzyme parameters | Specific activity (U/mg) (4 hrs) |
| Metal cofactors (1 mM) | Cu, Fe, and Mn | 90.6, 25, and 11.2 | Optimum metal cofactor | Cu | | Range of copper concentration tested | 0.05 mM–5 Mm | | Optimum Cu concentration | 0.7 mM | 96.87 | Range of NADH concentration | 0.01 mM–0.2 mM | | Optimum NADH concentration | 0.05 mM | 140.625 | Range of temperature | 4°C–42°C | | Optimum temperature | 36°C | 103.13 | Range of pH | 3–10 | | Optimum pH | 5 and 8 | 100 and 120 | Substrates | TCE, benzene, toluene, chloroform, hexane, and petroleum benzine | 87.5, 103.75, 31.87, 40.63, 13.75, and 11.25 | Optimum substrate | Benzene | 103.75 | Rate when benzene is the substrate |
Benzene | 340 U/mg/min | Rate when TCE is the substrate | TCE | 170 U/mg/min |
when benzene is the substrate | Benzene | 3.8 mM | when TCE is the substrate | TCE | 2.1 mM |
|
|