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BioMed Research International
Volume 2013 (2013), Article ID 724896, 8 pages
http://dx.doi.org/10.1155/2013/724896
Research Article

Establishment of a Lentiviral Vector Encoding Human HGF and the Infection of Human ADSCs

1Department of Hematology, Anhui Provincial Hospital of Anhui Medical University, Hefei 23001, China
2Department of Osteology, Anhui Provincial Hospital of Anhui Medical University, Hefei 23001, China

Received 11 October 2012; Revised 10 December 2012; Accepted 11 December 2012

Academic Editor: Irma Virant-Klun

Copyright © 2012 Xiaoyu Zhu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The delivery of adipose-derived stem cells (ADSCs) for promoting tissue repair has become a potential new therapy, while hepatocyte growth factor (HGF) is an important growth factor with angiogenic, anti-fibrotic, and anti-inflammatory benefits. In this paper, hADSCs were separated, cultured and identified based on the expression of cell surface antigens and multiple differentiation potential. We successfully generated a lentiviral vector encoding human HGF, infected hADSCs with this vector and examined the protein expression pattern. Finally we found that the hHGF lentiviral vector was successfully generated, and the lentiviral vector was able to safely infect hADSCs with high infection efficiency, thereby producing cells that overexpressed hHGF, which may provide a new strategy for the treatment of ischemic heart disease (IHD) and other ischemic diseases.