Immune Monitoring in Cancer Vaccine Clinical Trials: Critical Issues of Functional Flow Cytometry-Based Assays

Table 1

Critical issue		References
Blood collection, shipment, and processing	Temperature of storage	[16]
	Time from blood draw to sample processing	[17, 18]
	Freezing/thawing conditions	[19]
DNAse during culture		[20]
Perm/lysing reagents		[21]
Flow cytometric issues	Antibodies and fluorochromes	[22, 23]
	Spectral overlap and color compensation; Instrument setup
	Data acquisition and analysis	[24]