Table 3: Inhibitory effects of the compounds on the galactitol accumulation in rat lenses and erythrocytes.

CompoundsGalactitol content (μM)a in rat erythrocyte [inhibition %]Galactitol content μg/lens wet weight (g)b [inhibition %]

Galactose free0.98
Control22.80
Quercetin 16.15 [30.48%]
[46.82%]
Hirsutrin (6)15.70 [32.54%]
[33.78%]
3′-methoxyhirsutrin (7)20.19 [11.96%] [1.86%]

aErythrocyte was incubated in a Krebs-Ringer bicarbonate buffer containing 30 mM galactose and in the presence or absence of 50 μg/mL compounds. bMean standard deviation of triplication analysis of rat lens with compounds at a concentration 5 μg/mL. cQuercetin were used as positive control.