Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate

Table 2

Purification of xylanase from P. glabrum.

Purification step	Total activity; (U)	Total protein; (mg)	Specific activity; (U/mg protein)	Purification; (fold)	Recovery; (%)
Culture filtrate	2571.50	28.61	89.84	1.00	100.00
Supernatant 90% of (NH4)2SO4	2165.01	7.42	291.78	3.25	84.19
Sephadex G-75	1978.10	4.32	457.89	5.10	76.92