Production, Purification, and Characterization of a Major Penicillium glabrum Xylanase Using Brewer's Spent Grain as Substrate

Table 3

Effect of different substances on relative activity (%) of purified xylanase from P. glabrum.

	Relative activity (%)
Substance	Concentration
	2 mM	10 mM
Control	100	100
CuCl2	69.7 ± 0.3	21.3 ± 0.3
ZnSO4	84.4 ± 2.0	75.2 ± 1.1
MnSO4	178.9 ± 1.6	206.7 ± 2.2
BaCl2	117.5 ± 1.5	124.9 ± 1.7
CaCl2	122.0 ± 1.4	126.5 ± 1.2
NH4Cl	102.0 ± 1.55	134.3 ± 1.7
NaCl	99.3 ± 1.3	99.2 ± 1.5
SDS	ND	ND
PMSF	92.0 ± 0.9	89.3 ± 0.5
MgSO4	97.2 ± 1.7	97.0 ± 1.2
DTT	113.4 ± 1.2	134.4 ± 2.0
CoCl2	117.2 ± 1.6	122.2 ± 1.4
HgCl2	47.1 ± 0.5	ND
Pb(CH3COO)2	68.7 ± 2.0	19.1 ± 0.6
EDTA	89.0 ± 2.0	64.8 ± 1.0
𝛽-mercaptoethanol	124.0 ± 2.0	136.0 ± 2.0