αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors
Figure 2
αVβ5 and CD44 expressions in hESC. (a) Immunoflourescent staining of αVβ5 integrin and (b) CD44 (HCAM) in hESCs cultured in both 2% O2 and 21% O2. Scale Bar = 100 μm. Flow cytometry quantification of (c) αVβ5 integrin expression and (d) CD44 receptor expression, in hESCs cultured in both 2% and 21% O2 environments. (); *. -axis indicates % of hESCs with positive receptor expression. Black and open bars indicate 2% O2 and 21% O2, respectively. Error bars indicate standard deviations (SD).
