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BioMed Research International
Volume 2013 (2013), Article ID 729413, 15 pages
http://dx.doi.org/10.1155/2013/729413
Research Article

CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice

1Department of Biology, Medical College of Qingdao University, 38 Dengzhou Road, Qingdao 266021, China
2Department of Cardiology, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266100, China
3Department of Neurological Function Inspection, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266100, China

Received 5 July 2013; Revised 23 September 2013; Accepted 27 September 2013

Academic Editor: Senthil K. Venugopal

Copyright © 2013 Bing Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of C-phycocyanin (C-PC) on atherosclerosis and the regulatory effects of CD59 gene on anti-atherosclerotic roles of C-PC were investigated. Apolipoprotein E knockout (ApoE(−/−)) mice were randomly divided into four groups: control group, C-PC treatment group, CD59 transfection group and C-PC+CD59 synergy group. The mice were fed with high-fat-diet and treated with drug intervention at the same time. Results showed the atherosclerotic mouse model was successfully established. CD59 was over-expressed in blood and tissue cells. Single CD59 or C-PC could reduce blood lipid levels and promote the expression of anti-apoptotic Bcl-2 but inhibit pro-apoptotic Fas proteins in endothelial cells. The expression levels of cell cycle protein D1 (Cyclin D1) and mRNA levels of cyclin dependent protein kinase 4 (CDK4) in smooth muscle cells were restrained by CD59 and C-PC. CD59 or C-PC alone could inhibit the formation of atherosclerotic plaque by suppressing MMP-2 protein expression. In addition, C-PC could promote CD59 expression. So both CD59 and C-PC could inhibit the progress of atherosclerosis, and the anti-atherosclerotic effects of C-PC might be fulfilled by promoting CD59 expression, preventing smooth muscle cell proliferation and the apoptosis of endothelial cells, reducing blood fat levels, and at last inhibiting the development of atherosclerosis.