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BioMed Research International
Volume 2013 (2013), Article ID 734137, 15 pages
The CULTEX RFS: A Comprehensive Technical Approach for the In Vitro Exposure of Airway Epithelial Cells to the Particulate Matter at the Air-Liquid Interface
1Cultex Laboratories GmbH, Feodor-Lynen-Straße 21, 30625 Hannover, Germany
2Halter Engineering GmbH, Huebstraße 16, 9100 Herisau, Switzerland
3Von der Hardt 16, 57392 Oberkirchen, Germany
Received 8 October 2012; Revised 23 November 2012; Accepted 16 December 2012
Academic Editor: Abderrahim Nemmar
Copyright © 2013 Michaela Aufderheide et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- P. J. Barnes, “New therapies for chronic obstructive pulmonary disease,” Medical Principles and Practice, vol. 19, no. 5, pp. 330–338, 2010.
- A. D. Lopez and C. C. J. L. Murray, “The global burden of disease, 1990–2020,” Nature Medicine, vol. 4, no. 11, pp. 1241–1243, 1998.
- K. Bérubé, M. Aufderheide, D. Breheny et al., “In vitro models of inhalation toxicity and disease. The report of a FRAME workshop,” Alternatives to Laboratory Animals, vol. 37, no. 1, pp. 89–141, 2009.
- S. May, B. Jensen, M. Wolkenhauer, M. Schneider, and C. M. Lehr, “Dissolution techniques for in vitro testing of dry powders for inhalation,” Pharmaceutical Research, vol. 29, no. 8, pp. 2157–2166, 2012.
- B. M. Wright, “A new dust-feed mechanism,” Journal of Scientific Instruments, vol. 27, no. 1, pp. 12–15, 1950.
- C. Rovida and T. Hartung, “Re-evaluation of animal numbers and costs for in vivo tests to accomplish REACH legislation requirements for chemicals—a report by the transatlantic think tank for toxicology (t(4)),” ALTEX, vol. 26, no. 3, pp. 187–208, 2009.
- D. O. Raemy, R. N. Grass, W. J. Stark, C. M. Schumacher, M. J. Clift, et al., “Effects of flame made zinc oxide particles in human lung cells—a comparison of aerosol and suspension exposures,” Particle and Fibre Toxicology, vol. 9, article 33, 2012.
- L. K. Limbach, Y. Li, R. N. Grass et al., “Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations,” Environmental Science and Technology, vol. 39, no. 23, pp. 9370–9376, 2005.
- T. Hartung, “Thoughts on limitations of animal models,” Parkinsonism & Related Disorders, vol. 14, supplement 2, pp. S81–S83, 2008.
- B. K. Tarkington, R. Wu, W. M. Sun, K. J. Nikula, D. W. Wilson, and J. A. Last, “In vitro exposure of tracheobronchial epithelial cells and of tracheal explants to ozone,” Toxicology, vol. 88, no. 1–3, pp. 51–68, 1994.
- C. Voisin, C. Aerts, E. Jakubczak, J. L. Houdret, and T. B. Tonnel, “Effects of nitrogen dioxide on alveolar macrophages surviving in the gas phase. A new experimental model for the study of in vitro cytotoxicity of toxic gases (author's transl),” Bulletin Européen de Physiopathologie Respiratoire, vol. 13, no. 1, pp. 137–144, 1977.
- M. Aufderheide and U. Mohr, “CULTEX–a new system and technique for the cultivation and exposure of cells at the air/liquid interface,” Experimental and Toxicologic Pathology, vol. 51, no. 6, pp. 489–490, 1999.
- M. Aufderheide and U. Mohr, “A modified CULTEX system for the direct exposure of bacteria to inhalable substances,” Experimental and Toxicologic Pathology, vol. 55, no. 6, pp. 451–454, 2004.
- A. G. Lenz, E. Karg, B. Lentner, V. Dittrich, C. Brandenberger, et al., “A dose-controlled system for air-liquid interface cell exposure and application to zinc oxide nanoparticles,” Particle and Fibre Toxicology, vol. 6, article 32, 2009.
- K. de Bruijne, S. Ebersviller, K. G. Sexton et al., “Design and testing of Electrostatic Aerosol In Vitro Exposure System (EAVES): an alternative exposure system for particles,” Inhalation Toxicology, vol. 21, no. 2, pp. 91–101, 2009.
- M. Savi, M. Kalberer, D. Lang et al., “A novel exposure system for the efficient and controlled deposition of aerosol particles onto cell cultures,” Environmental Science and Technology, vol. 42, no. 15, pp. 5667–5674, 2008.
- M. Aufderheide, S. Scheffler, N. Mohle, B. Halter, and D. Hochrainer, “Analytical in vitro approach for studying cyto- and genotoxic effects of particulate airborne material,” Analytical and Bioanalytical Chemistry, vol. 401, no. 10, pp. 3213–3220, 2011.
- D. J. Giard, S. A. Aaronson, G. J. Todaro, et al., “In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors,” Journal of the National Cancer Institute, vol. 51, no. 5, pp. 1417–1423, 1973.
- M. Lieber, B. Smith, A. Szakal, et al., “A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells,” International Journal of Cancer, vol. 17, no. 1, pp. 62–70, 1976.
- F. Kohlrausch, Praktische Physik: zum Gebrauch für Unterricht, Forschung und Technik, V. Kose, S. Wagner, Ed., 24. Aufl., B. G. Teubner, Stuttgart, Germany, 1996.
- NanoCare, “Health related aspects of nanomaterials,” Project Report, 2009.
- B. Forbes, A. Shah, G. P. Martin, and A. B. Lansley, “The human bronchial epithelial cell line 16HBE14o- as a model system of the airways for studying drug transport,” International Journal of Pharmaceutics, vol. 257, no. 1-2, pp. 161–167, 2003.
- C. I. Grainger, L. L. Greenwell, D. J. Lockley, G. P. Martin, and B. Forbes, “Culture of Calu-3 cells at the air interface provides a representative model of the airway epithelial barrier,” Pharmaceutical Research, vol. 23, no. 7, pp. 1482–1490, 2006.
- A. Penn, G. Murphy, S. Barker, W. Henk, and L. Penn, “Combustion-derived ultrafine particles transport organic toxicants to target respiratory cells,” Environmental Health Perspectives, vol. 113, no. 8, pp. 956–963, 2005.
- B. Rothen-Rutishauser, F. Blank, C. Muhlfeld, and P. Gehr, “In vitro models of the human epithelial airway barrier to study the toxic potential of particulate matter,” Expert Opinion on Drug Metabolism and Toxicology, vol. 4, no. 8, pp. 1075–1089, 2008.
- A. C. Gray, J. D. McLeod, and R. H. Clothier, “A review of in vitro modelling approaches to the identification and modulation of squamous metaplasia in the human tracheobronchial epithelium,” Alternatives to Laboratory Animals, vol. 35, no. 5, pp. 493–504, 2007.
- M. Aufderheide, “Direct exposure methods for testing native atmospheres,” Experimental and Toxicologic Pathology, vol. 57, supplement 1, pp. 213–226, 2005.
- M. Aufderheide, “An efficient approach to study the toxicological effects of complex mixtures,” Experimental and Toxicologic Pathology, vol. 60, no. 2-3, pp. 163–180, 2008.
- M. Aufderheide and U. Mohr, “CULTEX—an alternative technique for cultivation and exposure of cells of the respiratory tract to airborne pollutants at the air/liquid interface,” Experimental and Toxicologic Pathology, vol. 52, no. 3, pp. 265–270, 2000.
- E. Bitterle, E. Karg, A. Schroeppel et al., “Dose-controlled exposure of A549 epithelial cells at the air-liquid interface to airborne ultrafine carbonaceous particles,” Chemosphere, vol. 65, no. 10, pp. 1784–1790, 2006.
- Y. Fukano, M. Ogura, K. Eguchi, M. Shibagaki, and M. Suzuki, “Modified procedure of a direct in vitro exposure system for mammalian cells to whole cigarette smoke,” Experimental and Toxicologic Pathology, vol. 55, no. 5, pp. 317–323, 2004.
- S. Mülhopt, H. R. Paur, S. Diabate, and H. F. Krug, “In-vitro testing of inhalable fly ash at the air liquid interface,” in Environmental Monitoring, Y. J. Kim, Ed., pp. 402–414, Springer, Dordrecht, The Netherlands, 2008.
- B. Rothen-Rutishauser, F. Blank, C. Mühlfeld, and P. Gehr, “Nanoparticle-cell membrane interactions,” in Particle-Lung Interactions, P. Gehr, C. Mühlfeld, B. Rothen-Rutishauser, and F. Blank, Eds., pp. 226–242, Informa Healtscare, New York, NY, USA, 2009.
- B. Rothen-Rutishauser, R. N. Grass, F. Blank et al., “Direct combination of nanoparticle fabrication and exposure to lung cell cultures in a closed setup as a method to simulate accidental nanoparticle exposure of humans,” Environmental Science and Technology, vol. 43, no. 7, pp. 2634–2640, 2009.
- J. Phillips, B. Kluss, A. Richter, and E. D. Massey, “Exposure of bronchial epithelial cells to whole cigarette smoke: assessment of cellular responses,” Alternatives to Laboratory Animals, vol. 33, no. 3, pp. 239–248, 2005.
- J. Adamson, D. Azzopardi, G. Errington, C. Dickens, J. McAughey, et al., “Assessment of an in vitro whole cigarette smoke exposure system: the Borgwaldt RM20S 8-syringe smoking machine,” Chemistry Central Journal, vol. 5, article 50, 2011.
- A. Tippe, U. Heinzmann, and C. Roth, “Deposition of fine and ultrafine aerosol particles during exposure at the air/cell interface,” Journal of Aerosol Science, vol. 33, no. 2, pp. 207–218, 2002.
- H. L. Karlsson, J. Gustafsson, P. Cronholm, and L. Möller, “Size-dependent toxicity of metal oxide particles—a comparison between nano- and micrometer size,” Toxicology Letters, vol. 188, no. 2, pp. 112–118, 2009.
- K. Donaldson and C. L. Tran, “Inflammation caused by particles and fibers,” Inhalation Toxicology, vol. 14, no. 1, pp. 5–27, 2002.
- G. Oberdörster, “Pulmonary effects of inhaled ultrafine particles,” International Archives of Occupational and Environmental Health, vol. 74, no. 1, pp. 1–8, 2001.
- S. Park, Y. K. Lee, M. Jung et al., “Cellular toxicity of various inhalable metal nanoparticles on human alveolar epithelial cells,” Inhalation Toxicology, vol. 19, supplement 1, pp. 59–65, 2007.
- J. M. Veranth, E. G. Kaser, M. M. Veranth, M. Koch, and G. S. Yost, “Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts,” Particle and Fibre Toxicology, vol. 4, article 2, 2007.
- D. B. Warheit, T. R. Webb, V. L. Colvin, K. L. Reed, and C. M. Sayes, “Pulmonary bioassay studies with nanoscale and fine-quartz particles in rats: toxicity is not dependent upon particle size but on surface characteristics,” Toxicological Sciences, vol. 95, no. 1, pp. 270–280, 2007.
- M. Ahamed, M. A. Siddiqui, M. J. Akhtar, I. Ahmad, A. B. Pant, and H. A. Alhadlaq, “Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells,” Biochemical and Biophysical Research Communications, vol. 396, no. 2, pp. 578–583, 2010.
- H. L. Karlsson, P. Cronholm, J. Gustafsson, and L. Möller, “Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes,” Chemical Research in Toxicology, vol. 21, no. 9, pp. 1726–1732, 2008.
- D. Ritter, J. W. Knebel, and M. Aufderheide, “In vitro exposure of isolated cells to native gaseous compounds—development and validation of an optimized system for human lung cells,” Experimental and Toxicologic Pathology, vol. 53, no. 5, pp. 373–386, 2001.
- J. W. Knebel, D. Ritter, and M. Aufderheide, “Exposure of human lung cells to native diesel motor exhaust—development of an optimized in vitro test strategy,” Toxicology in Vitro, vol. 16, no. 2, pp. 185–192, 2002.
- S. Diabaté, S. Mülhopt, H. R. Paur, and H. F. Krug, “The response of a co-culture lung model to fine and ultrafine particles of incinerator fly ash at the air-liquid interface,” Alternatives to Laboratory Animals, vol. 36, no. 3, pp. 285–298, 2008.
- F. Pariselli, M. G. Sacco, and D. Rembges, “An optimized method for in vitro exposure of human derived lung cells to volatile chemicals,” Experimental and Toxicologic Pathology, vol. 61, no. 1, pp. 33–39, 2009.
- U. Deschl, J. Vogel, and M. Aufderheide, “Development of an in vitro exposure model for investigating the biological effects of therapeutic aerosols on human cells from the respiratory tract,” Experimental and Toxicologic Pathology, vol. 63, no. 6, pp. 593–598, 2011.
- C. Schmalz, H. G. Wunderlich, R. Heinze, F. H. Frimmel, C. Zwiener, et al., “Application of an optimized system for the well-defined exposure of human lung cells to trichloramine and indoor pool air,” Journal of Water and Health, vol. 9, no. 3, pp. 586–596, 2011.
- D. Wijte, M. J. Alblas, D. Noort, J. P. Langenberg, and H. P. van Helden, “Toxic effects following phosgene exposure of human epithelial lung cells in vitro using a CULTEX system,” Toxicology in Vitro, vol. 25, no. 8, pp. 2080–2087, 2011.
- H. Nara, Y. Fukano, T. Nishino, and M. Aufderheide, “Detection of the cytotoxicity of water-insoluble fraction of cigarette smoke by direct exposure to cultured cells at an air-liquid interface,” Experimental and Toxicologic Pathology. In press.
- K. Elihn, P. Cronholm, H. L. Karlsson, K. Midander, I. Odnevall Wallinder, et al., “Cellular dose of partly soluble Cu particle aerosols at the air-liquid interface using an in vitro lung cell exposure system,” Journal of Aerosol Medicine and Pulmonary Drug Delivery. In press.
- A. Gaschen, D. Lang, M. Kalberer et al., “Cellular responses after exposure of lung cell cultures to secondary organic aerosol particles,” Environmental Science and Technology, vol. 44, no. 4, pp. 1424–1430, 2010.
- T. Tang, R. Gminski, M. Konczol, C. Modest, B. Armbruster, et al., “Investigations on cytotoxic and genotoxic effects of laser printer emissions in human epithelial A549 lung cells using an air/liquid exposure system,” Environmental and Molecular Mutagenesis, vol. 53, no. 2, pp. 125–135, 2012.
- E. Frohlich, G. Bonstingl, A. Hofler, C. Meindl, G. Leitinger, et al., “Comparison of two in vitro systems to assess cellular effects of nanoparticles-containing aerosols,” Toxicology in Vitro, vol. 27, no. 1, pp. 409–417, 2012.
- S. E. Anderson, L. G. Jackson, J. Franko, and J. R. Wells, “Evaluation of dicarbonyls generated in a simulated indoor air environment using an in vitro exposure system,” Toxicological Sciences, vol. 115, no. 2, pp. 453–461, 2010.
- R. Gminski, K. Decker, C. Heinz et al., “Genotoxic effects of three selected black toner powders and their dimethyl sulfoxide extracts in cultured human epithelial A549 lung cells in vitro,” Environmental and Molecular Mutagenesis, vol. 52, no. 4, pp. 296–309, 2011.
- J. Volckens, L. Dailey, G. Walters, and R. B. Devlin, “Direct particle-to-cell deposition of coarse ambient particulate matter increases the production of inflammatory mediators from cultured human airway epithelial cells,” Environmental Science and Technology, vol. 43, no. 12, pp. 4595–4599, 2009.