Delivery of RNA and Its Intracellular Translation into Protein Mediated by SDS-CTAB Vesicles: Potential Use in Nanobiotechnology

Figure 4

Transfection efficiency of mRNA CAT after treatment with RNase. In this experiment, the mRNA CAT was added to the surfactant mixture prior to the vesicle formation. This result strongly suggests that the RNA is internalized within the vesicle aqueous space and is thus protected by the nucleolytic attack. Panel (A): vesicles not treated with RNase (last two bars to the right) exhibit a higher efficiency than Lipofectamine. Panel (B): comparison between RNase untreated and treated vesicles. The numbers at the bottom of the bar indicate the vesicle concentration (μM).