TrkAIII Promotes Microtubule Nucleation and Assembly at the Centrosome in SH-SY5Y Neuroblastoma Cells, Contributing to an Undifferentiated Anaplastic Phenotype
Figure 4
(a) IP/Western blots demonstrating differences in α-tubulin levels pulled down by TrkAIII and TrkAI immunoprecipitates from respective SH-SY5Y transfectants, plus histograms displaying densitometric analysis of the adjacent blots, demonstrating the presence of similar levels of TrkAI and TrkAIII immunoprecipitates, normalised to input α-tubulin levels, plus the difference in α-tubulin levels pulled down as a densitometric ratio to TrkAI and TrkAIII. (b) IP/Western blots demonstrating the effect of CEP-701 (100 nM for 0–16 hours) on TrkAIII tyrosine phosphorylation (P-Tyr) and α-tubulin levels pulled down in coimmunoprecipitation assays, plus histograms displaying densitometric analysis of the adjacent blots, demonstrating CEP-701-induced loss of TrkAIII tyrosine phosphorylation from 3 hr onwards, associated with reduced α-tubulin binding at 16 hours only.
