Hypoxia and Human Genome Stability: Downregulation of BRCA2 Expression in Breast Cancer Cell Lines
Figure 2
Heat maps of DEGs involved in DNA damage repair (DDR) pathways deregulated in hypoxia. Clustering of DEGs involved in DNA damage repair (DDR) pathways: mismatch repair (MMR), nucleotide excision repair (NER), nonhomologous end-joining (NHEJ), and homologous recombination repair (HRR). The heat maps were generated from microarray data reflecting gene expression values in MCF-7, MDA-MB-231, and SKBr3 cells exposed to hypoxia (3% O2) for 24 h and 48 h in comparison to control cells cultured under normoxic conditions (16% O2, and ). Each row represents the expression levels for a single gene tested for different experimental conditions. Each column shows the expression levels for the genes tested for a single experimental condition. The absolute expression value (log scale) of each gene is derived from the mean of two biological replicates. The color scale bar on the top represents signal intensity variations ranging from green (poorly expressed genes) to red (highly expressed genes). Black boxes indicate intermediate expression values. N = normoxia; H24 = hypoxia for 24 h; H48 = hypoxia for 48 h.
