Hypoxia and Human Genome Stability: Downregulation of BRCA2 Expression in Breast Cancer Cell Lines
Figure 3
Effects of hypoxia on BRCA2 expression in breast cancer cell lines. Validation of microarray data by quantitative real-time PCR analysis. Changes in BRCA1 (a), BRCA2 (b), and MLH1 (c) mRNA expression levels were determined in MCF-7, SKBr3, and MDA-MB-231 cells exposed to hypoxia (3% O2) for 24 and 48 hours with respect to control condition (normoxia, 16% O2). Relative transcript levels were determined using the method and normalized to β-actin mRNA (endogenous control). Normoxic condition values are taken as 1 and hypoxic condition values represent fold decrease relative to control condition. Data are presented as fold changes ± SDs. Significant difference, hypoxia for 24 h (H24) or for 48 h (H48) versus normoxia (N), .
