Research Article
Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons
Table 1
Sequences for qPCR primers.
| | Gene name | Gene ID | Forward primer | Reverse primer |
| | 18S ribosomal RNA | 18S rRNA | CCCGAAGCGTTTACTTTGAA | CCCTCTTAATCATGGCCTCA | | Dopamine transporter (DAT) | Slc6a3 | GCCCATTTATGCGACCTACA | GATGGTCTTTCTCAGGTGTGATG | | Tyrosine hydroxylase | TH | TGTGTCCGAGAGCTTCAATG | GCTGGATACGAGAGGCATAGTTC | | Glial fibrillary acidic protein | Gfap | CAACCTCCAGATCCGAGAAA | GCTCCTGCTTCGACTCCTTA | | Nuclear orphan receptor (Nurr1) | Nr4a2 | CGATCAGGACCTGCTTTTTG | CTGGGTTGGACCTGTATGCT | | Vesicular monoamine transporter 2 (Vmat2) | Slc18a2 | AGACCATGTGTTCCCGAAAG | CACATAGCCACCTTCCCATT | | Glutamic acid decarboxylase 65 (GAD65) | Gad2 | TTGCGAGTTCTGGAAGACAA | AGATGACCATGCGGAAGAAG | | Vesicular glutamate transporter 2 (VGlut2) | Slc17a6 | GATATTGCCCCGAGATATGC | CCATTCTTCACGGGACTTGT |
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