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Figure 4: Growth curve of Leishmania major promastigotes in RPMI-1640 medium plus 10% fetal calf serum (FCS) and RPMI-1640 medium plus 10% filtered sheep urine (F.Sh.Ur), filtered mouse urine (F.M.Ur), autoclaved sheep urine (A.Sh.Ur), and autoclaved mouse urine (A.M.Ur).