Optimization of Polygalacturonase Production from a Newly Isolated Thalassospira frigidphilosprofundus to Use in Pectin Hydrolysis: Statistical Approach
Table 10
(a) Ranges and levels of the factors for CCD matrix in pectin hydrolysis. (b) Experimental design and result of hydrolysis based on pectin yielded (%) and reducing sugar level.
(a)
Factors
Symbol
Coded level
−2
−1
0
1
2
Volume of crude enzyme extract (mL)
A
15
2025
35
40
Temperature (°C)
B
10
15
20
25
30
Amount of banana fiber (mg)
C
25
3035
40
45
Incubation time (min)
D
30
4590
120
150
(b)
Sl. No.
A
B
C
D
Pectin yield (%)
Reducing sugar (mg/mL)
†Experimental valuea CCD predicated
†Experimental valueb CCD predicated
1
−1
−1
−1
−1
2.67
0.08
2
1
−1
−1
−1
1.58
0.24
3
−1
1
−1
−1
2.98
0.10
4
1
1
−1
−1
1.73
0.19
5
−1
−1
1
−1
3.17
0.05
6
1
−1
1
−1
2.14
0.18
7
−1
1
1
−1
4.06
0.06
8
1
1
1
−1
2.87
0.12
9
−1
−1
−1
1
3.07
0.08
10
1
−1
−1
1
1.70
0.24
11
−1
1
−1
1
3.68
0.08
12
1
1
−1
1
2.14
0.18
13
−1
−1
1
1
2.17
0.14
14
1
−1
1
1
0.86
0.38
15
−1
1
1
1
3.36
0.14
16
1
1
1
1
1.89
0.21
17
−2
0
0
0
4.37
0.01
18
2
0
0
0
1.81
0.24
19
0
−2
0
0
2.82
0.10
20
0
2
0
0
4.16
0.05
21
0
0
−2
0
1.63
0.19
22
0
0
2
0
1.87
0.19
23
0
0
0
−2
1.98
0.16
24
0
0
0
2
1.40
0.25
25
0
0
0
0
1.48
0.12
26
0
0
0
0
1.48
0.12
27
0
0
0
0
1.48
0.12
28
0
0
0
0
1.48
0.12
29
0
0
0
0
1.48
0.12
30
0
0
0
0
1.48
0.12
R-sq = 98.40% and bR-sq = 86.71%. †Experimental values are mean of triplicates test.
