Increasing Affinity of Interferon-𝜸 Receptor 1 to Interferon-𝜸 by Computer-Aided Design

Figure 2

Conserved residues calculated by strict alignment of 32 sequences of the extracellular part of IFN-𝛾 receptor 1 from 19 species. The receptor residues forming the interface with IFN-𝛾 (i.e., residues no further than 6 Å from an IFN-𝛾 atom) are highlighted in yellow; the residues selected for mutations are in red. All the selected mutations are listed in Table 2. Percentages of the conservation are shown on the left and right sides, sequence and numbering of UniProt P15260 on the bottom. Sequences used for the alignment are listed in Table S1. Numbering of the PDB entry 1fg9 can be derived from the UniProt one by subtracting 17. The alignment was computed by KAlign [18] as implemented in program Ugene [19].