Increasing Affinity of Interferon- Receptor
1 to Interferon- by Computer-Aided Design
Figure 4
Association and dissociation curves of the SPR experiments. Most IFN--Rx variants behave similarly as the wild-type (a) and mutant H222R (b): they bind IFN--SC very fast but also release it fast. Two high-affinity binders, mutant N96W (c) and the double mutant N96W+H222R, bind the IFN--SC molecules for a longer time, thus increasing the affinity to IFN-. The SPR experimental signal is in black; the fitted curves from which the association and dissociation kinetic constants are calculated are in red. The SPR data for all variants are in Table 3.
