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BioMed Research International
Volume 2013 (2013), Article ID 752514, 12 pages
Increasing Affinity of Interferon- Receptor 1 to Interferon- by Computer-Aided Design
Institute of Biotechnology AS CR, v. v. i., Vídeňská 1083, 142 20 Prague, Czech Republic
Received 29 April 2013; Revised 6 August 2013; Accepted 13 August 2013
Academic Editor: David Stammers
Copyright © 2013 Pavel Mikulecký et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Supplementary material consists of two text sections, four tables, and one figure:
Supplementary text contains i) a short protocol for the computational procedures used, and ii) statistical treatment of the SPR experiments, mainly evaluation of significance of obtained Kd values and discussion of the influence of different batches of IFN-γ on measured Kd values.
Supplementary Table S1 lists GenBank IDs of sequences used in global alignment of receptor sequences, Table S2 mutagenesis primers for construction of IFN-γ-Rx mutants, Table S3 lists FoldX-calculated ΔΔG values, and Table S4 temperature dependencies of Kd.
Supplementary Figure S1 displays melting curves of WT and selected mutants.