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Figure 4: Identification of the recombinant pVAX1-sFLT-1 plasmid. Lane M: DNA marker (1 Kb plus 100 bp, Mbiotech, Korea); lane 1: digestion of plasmid pVAX1 (negative control) with Hind III (3000 bp); lane 2: digestion of recombinant plasmid with Hind III (4000 bp); lane 3: digestion of recombinant plasmid with Hind III+ ECORI, arrow indicates release of sFLT-1 fragment (1000 bp).