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Figure 6: Secretion of sFLT-1 in conditioned media of pVAX1-sFLT-1 transfected cells. (a) Western blot analysis with anti-mouse VEGFR-1 antibody, similar results were observed as arrow indicates. Lane M: (PageRuler Prestained Protein Ladder, Fermentas, Lithuania); lanes 1 and 2: K562 and HL60 cells transfected with pVAX1-sFLT-1; lanes 3 and 4: K562 and HL60 cells transfected with pVAX1-hMGFP (negative control).