An Experimental Approach for Selecting Appropriate Rodent Diets for Research Studies on Metabolic Disorders

Table 2

Effect of different experimental diets on biochemical parameters in male Wistar rats.

Treatment groups	Serum triglyceride (mg/dL)			Serum total cholesterol (mg/dL)			Serum glucose (mg/dL)
	Day 0	Day 42	Day 84	Day 0	Day 42	Day 84	Day 0	Day 42	Day 84
I; NPD	91.18±7.94	120.55±9.45	148.24±5.38	67.54±2.31	60.14±2.31	63.51±2.56	85.85±2.92	92.03±3.70	102.66±8.35
II; NCD-I	99.80±6.97	116.26±7.53	126.19±9.63	64.99±2.71	66.26±4.35	64.29±4.49	88.49±3.94	99.40±5.36	100.60±3.92
III; NCD-II	98.78±8.67	148.87±9.21	177.90±14.51	62.57±2.14	64.43±3.39	66.11±4.23	92.87±2.82	102.20±5.76	102.70±2.94
IV; NCD-III	98.13±4.74	154.12±8.80	188.92±13.20^b	66.41±2.11	68.55±2.24	68.53±3.97	85.34±3.09	101.78±3.19	105.55±4.64
V; HFD-I	91.88±8.54	193.68±11.91^ab	248.97±19.26^ab	67.42±4.80	83.41±3.02^abcd	91.47±1.89^abcd	90.24±4.08	109.09±5.49	119.24±11.42
VI; HFD-II	99.49±5.84	182.85±13.28^ab	238.66±21.65^ab	69.07±2.46	80.48±3.01^ac	80.34±1.75^ab	87.56±3.10	106.54±8.39	115.84±9.30
VII; HFD-III	97.92±5.10	188.13±21.28^ab	279.21±31.11^abc	68.54±2.70	87.09±4.03^abcd	89.00 ±4.03^abcd	93.46±2.32	116.35±6.45	125.34±9.27

Values are expressed as mean ± SEM; 𝑛=8.
^a𝑃≤0.05 versus NPD, ^b𝑃≤0.05 versus NCD-I, ^c𝑃≤0.05 versus NCD-II, and ^d𝑃≤0.05 versus NCD-III.
NPD: normal pellet diet, NCD: normal control diet, and HFD: high fat diet.