High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli

Table 1

Primers and restriction enzymes used for OG2 amplification and vector construction.

Genes	Primers	Sequence 5′-3′	Restriction enzyme
C-OG2; (C-terminal fusion)	PC1	GGGAATTCCATATGAAGAAATTCTTCCTGAAAGT; GCT	Nde I
	PC2	CCCGCCACTTTGCAGCGAATTTTGGTCAGCACTT; TCAGGAAGAAT
	PC3	GGACTAGTGCATCTCCCGTGATGCAGGTACGAC; AGCCACCCGCCACTTTGCAG	Spe I
N-OG2; (N-terminal fusion)	PN1	CATAACTTTGTCGCGAATGACATCATTGTACACA; ACAAGAAATTCTTCCTGAAAGTGCT	Nru I
	PN2	CCCGCCACTTTGCAGCGAATTTTGGTCAGCACTT; TCAGGAAGAAT
	PN3	CGCGGATCC TTAGGTACGACAGCCACCCGCCAC; TTTGCAG	Bam HI