Research Article

High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli

Table 2

Growth condition optimization of for the C-terminal fusion and N-terminal fusion strains.

StrainsTemperature (°C)IPTG (mM)Induction period (h)

BL21-OG2-intein2370.52
370.12
300.12

BL21-intein1-OG2370.52
370.12
300.12
200.16