High Glucose-Induced Oxidative Stress Increases the Copy Number of Mitochondrial DNA in Human Mesangial Cells
Figure 2
High glucose-induced ROS generation. Synchronized quiescent mesangial cells grown on glass coverslips were incubated for 24 h with 5 mM glucose or 25 mM and loaded with carboxy-H2-DCFDA (25 μM, 30 min). Positive control cells were incubated with 5 mM glucose, and treated with TBHP (100 μM, 1 h), washed and loaded with carboxy-H2-DCFDA (25 μM, 30 min). Intracellular ROS was detected by confocal microscopy and flow cytometry. (a) Representative confocal images of ROS generation in human mesangial cells. The fluorescent intensity of DCF corresponds to the extent of ROS accumulation. (b) Representative histograms of flow cytometry demonstrating the fluorescent intensity of DCF in human mesangial cells ().
