Table 3: Cytochrome P450, EMND, and AH activities measured in rat liver microsomes.

GroupEMND activity nmol/mg proteinAH activity nmol/mg proteinCytochrome P450 nmol/mg protein

Control0.486 ± 0.0320.075 ± 0.0030.325 ± 0.04
Paracetamol0.467 ± 0.020.078 ± 0.0050.345 ± 0.04
Saponarin0.371 ± 0.011*0.047 ± 0.004*0.244 ± 0.05*
PC + SP0.375 ± 0.006*+0.048 ± 0.005*+0.236 ± 0.03*+
Silymarin0.340 ± 0.013*0.051 ± 0.005*0.255 ± 0.03*
PC + SL0.346 ± 0.02*+0.048 ± 0.004*+0.262 ± 0.02*+

Treatment: paracetamol administered at a dose of 600 mg/kg i.p alone and two hours after the last administration of the hepatoprotective agents (SP + PC and SL + PC groups); saponarin (SP) (80 mg/kg bw/day) and silymarin (SL) (100 mg/kg bw/day) were administered p.o. for 7 days. Data are expressed as mean ± SEM of six rats. *Significant difference from control values (Mann-Whitney test, ). +Significant difference from paracetamol-treated group (Mann-Whitney test, ).