Exploring the Possible Role of Lysine Acetylation on Entamoeba histolytica Virulence: A Focus on the Dynamics of the Actin Cytoskeleton
Figure 3
Aspirin affects the dynamics of the actin cytoskeleton and its polymerization in live trophozoites. (a) Confocal microscopy analysis of trophozoites treated or not with 1 mM Aspirin. Actin was stained with rhodamine-phalloidin (red) (1 : 25) and nucleus with DAPI (blue) (1 : 300). (b) FN-adhered trophozoites, treated or not with Aspirin for 2 h, were extracted with 0.5% Triton X-100 and separated into 8,000 ×g supernatant and pellet fractions, where actin was detected by western blot with antiactin antibody (1 : 5000).
