Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods
Table 1
ALK positive cases detected by FISH, IHC, real-time RT-PCR, and targeted resequencing.
Sample ID
Age
Sex
Smoking
FISH
IHC
RT-PCR
Targeted resequencing
Fusion type/% of positive cells
Variant type
Fusion/break position in ALK
LC1
46
F
Nonsmoker
Single-signal/70
Strongly positive
EML4-ALK variant 1/2/3a/3b
chr2: 29447508–29447608
LC11
48
M
Nonsmoker
Pair-signal/46
Strongly positive
EML4-ALK variant 1/2/3a/3b
chr2: 29446498–29446566
LC14*
44
M
Nonsmoker
Pair-signal/74
Not studied
EML4-ALK variant 1/2/3a/3b
chr2: 29446561–29446661
LC74*
44
M
Nonsmoker
Pair-signal/46
Strongly positive
EML4-ALK variant 1/2/3a/3b
chr2: 29446561–29446661
LC51
51
M
Nonsmoker
Single-signal/66
Strongly positive
EML4-ALK variant 1/2/3a/3b
chr2: 29446527–29446625
15915
63
M
Nonsmoker
Single-signal/40
Strongly positive
EML4-ALK variant 1/2/3a/3b
Not studied
*LC14 and LC74 are samples from the same patient but from a different FFPE block.
