Silencing of Entamoeba histolytica Glucosamine 6-Phosphate Isomerase by RNA Interference Inhibits the Formation of Cyst-Like Structures
Figure 2
Levels of E. histolytica Gln6Pi protein expression in trophozoites transfected with different amounts of a mix of si 154-Gln6Pi and si 229-Gln6Pi determined by western blot. Trophozoites (about cells) were tranfected with siRNAs mix at indicated amounts by soaking during 16 h at 37°C. Afterwards, total extracts were prepared in the presence of protease inhibitors, run in SDS-PAGE, and transferred to nitrocellulose paper. Gln6Pi (37 kDa) was identified by using a mouse anti-human Gln6Pi polyclonal antibody and revealed using ECL. Relative expression was determined by densitometry taking basal expression of Gln6Pi in untreated trophozoites as 100 percent (CTR (+)). CTR (siRNA): trophozoites transfected with scrambled-sequence siRNA-A. The amount of protein loaded in each lane is shown in a Coomassie stained gel.
