Silencing of Entamoeba histolytica Glucosamine 6-Phosphate Isomerase by RNA Interference Inhibits the Formation of Cyst-Like Structures

Table 1

Oligonucleotides and siRNAs used in this work for RT-PCR and interference of Gln6Pi in E. histolytica, respectively.

Target gene	Primer name	Sequence (5′ to 3′)
gln6Pi	Gln6PiEh-F	ATGTCATCCACAAACGAAAATATTC
gnl6Pi	Gln6PiEh-R	CAATAGACATGGATTTATCATATC
EhARF	EhARF-F	GTAGGACTTGATGCTGCC
EhARF	EhARF-R	TCACCATTAGTTGCAC
gln6Pi-mRNA	siRNA 154-Gln6Pi	GGACAUGCAGUAUUAGGAUTT
gln6Pi-mRNA	Si 229-Gln6Pi	GCUGGAGAAGUUUCAUUUATT
—	siRNA-A:sc37007	—

Controls for constitutive expression (ARF primers) and specificity of interference (siRNA-A) are also shown.